Composite

Part:BBa_K3003010:Design

Designed by: Aysenur Deniz Cayirtepe   Group: iGEM19_Bilkent-UNAMBG   (2019-10-16)


PelB - PenetraMax - Wild Type Insulin with a Linker Peptide - Ag43


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 268
    Illegal PstI site found at 646
    Illegal PstI site found at 1204
    Illegal PstI site found at 1804
    Illegal PstI site found at 2428
    Illegal PstI site found at 2699
    Illegal PstI site found at 2752
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 268
    Illegal PstI site found at 646
    Illegal PstI site found at 1204
    Illegal PstI site found at 1804
    Illegal PstI site found at 2428
    Illegal PstI site found at 2699
    Illegal PstI site found at 2752
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 100
    Illegal XhoI site found at 256
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 268
    Illegal PstI site found at 646
    Illegal PstI site found at 1204
    Illegal PstI site found at 1804
    Illegal PstI site found at 2428
    Illegal PstI site found at 2699
    Illegal PstI site found at 2752
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 268
    Illegal PstI site found at 646
    Illegal PstI site found at 1204
    Illegal PstI site found at 1804
    Illegal PstI site found at 2428
    Illegal PstI site found at 2699
    Illegal PstI site found at 2752
    Illegal NgoMIV site found at 54
    Illegal NgoMIV site found at 472
    Illegal NgoMIV site found at 1696
    Illegal NgoMIV site found at 2206
    Illegal NgoMIV site found at 2227
    Illegal AgeI site found at 1222
    Illegal AgeI site found at 1966
    Illegal AgeI site found at 2380
    Illegal AgeI site found at 2622
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.


Source

Cloned from its components.

References

Chance, R. E., Dimarchi, R. D., Hoffmann, J. A., Long, H. B., & Miller, A. R. (n.d.).